Clinical value of circular RNA hsa_circ_0133159 in diagnosis and prognostic evaluation of glioma

HU Xiang; YIN Xiaoming; WANG Donglei

doi:10.12022/jnnr.2017-0079

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Journal of Neurology and Neurorehabilitation ›› 2017, Vol. 13 ›› Issue (4) : 165-172. DOI: 10.12022/jnnr.2017-0079

Original Research

Clinical value of circular RNA hsa_circ_0133159 in diagnosis and prognostic evaluation of glioma

HU Xiang, YIN Xiaoming, WANG Donglei

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Abstract

Objective: To investigate the clinical value of circular RNA hsa_circ_0133159 in diagnosis and prognostic evaluation of glioma. Methods: The relative expressions of hsa_circ_0133159 in 10 normal brain tissue samples and 38 glioma tissue samples were detected by real-time fluorescence-based quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and its relationship with clinicopathological characteristics was analyzed. The clinical value of hsa_circ_0133159 in diagnosis of glioma was evaluated by using the receiver operating characteristic (ROC) curve. The survival analysis was conducted using Kaplan-Meier method, and the univariate analysis of prognostic factors was conducted by using log-rank test. The multivariate analysis was conducted by using COX regression analysis. Results: Compared with normal brain tissues, the relative expressions of hsa_circ_0133159 in low-grade and high-grade glioma were increased significantly (P = 0.026, P = 0.007). The area under curve (AUC) of hsa_circ_0133159 in ROC curve was 0.887 and its sensitivity and specificity for the diagnosis of glioma were 86.8% and 80.0%, respectively with the cut-off value of 4.32. The progression-free survival (PFS) and overall survival (OS) of glioma patients with low expression of hsa_circ_0133159 (≤4.32, n = 17) were significantly prolonged as compared with those of glioma patients with high expression of hsa_circ_0133159 (> 4.32, n = 21). The result of univariate analysis showed that the Karnofsky performance status (KPS) score, number of tumor lesions, pathologic grade, recurrence and the relative espression of hsa_circ_0133159 were significantly associated with PFS and OS of patients with glioma (P < 0.05)。Multivariate analysis revealed that relative expression of hsa_circ_0133159 was an independent prognostic factor of glioma [PFS: relative risk = 1.268 (95% confidence interval: 1.007-3.459), P = 0.001; OS: relative risk = 1.252 (95% confidence interval: 1.103-2.422), P = 0.001]. Conclusion: hsa_circ_0133159 may be used as a new biomarker for the diagnosis and prognostic evaluation of glioma.

Key words

Glioma / Circular RNA / hsa_circ_0133159 / Receiver operating characteristic curve / Diagnosis / Prognosis

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HU Xiang, YIN Xiaoming, WANG Donglei. Clinical value of circular RNA hsa_circ_0133159 in diagnosis and prognostic evaluation of glioma[J]. Journal of Neurology and Neurorehabilitation. 2017, 13(4): 165-172 https://doi.org/10.12022/jnnr.2017-0079

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